中国生物防治学报Issue(5)：678-684,7.
武夷菌素部分生物合成基因簇的克隆和分析
Cloning and Analysis of Wuyiencin Partial Biosynthetic Gene Cluster of Streptomyces ahygroscopicus var. wuyiensis CK-15
摘要
Abstract
A wuyiencin producing strain Streptomyces ahygroscopicus var. wuyiensis CK-15 was isolated and purified from Wuyi mountain soil in Fujian province. Wuyiencin as secondary metabolites has good control effect on fruit and vegetable fungal diseases whereas which is limited on its low production disadvantage in industrial production and agricultural application. In order to achieve the aim of breeding high yield strain by genetic engineering, an attempt to obtain the biosynthetic gene cluster of wuyiencin generated strain was made. In this study, primers were designed according to a sequence of macrolide antibiotic polyketone synthetase gene, which was used for screening CK-15 genomic library. Nine positive clones were identified from the Streptomyces ahygroscopicus var. wuyiensis CK-15 fosmid genomic library. The positive clones were sequenced. There were three large scaffolds with approximately 53.291 kb of gene sequence. This sequence contains 14 possible ORFs and show high homology with nystatin biosynthetic gene of S. noursei ATCC 11455. The research will facilitate the further functional analysis of the wuyiencin biosynthetic genes and breed high yield new strain through genetic engineering.关键词
武夷菌素/生物合成基因簇/克隆/分析Key words
wuyiencin/biosynthetic gene cluster/clone/analysis分类
农业科技引用本文复制引用
葛蓓孛,杨振娟,檀贝贝,刘彦彦,刘艳,孙蕾,张克诚..武夷菌素部分生物合成基因簇的克隆和分析[J].中国生物防治学报,2014,(5):678-684,7.基金项目
公益性行业（农业）科研专项（201103002，201303025）；国家自然科学基金 （）
