中国生物防治学报Issue(6)：731-735,5.
利用改良的Chelex方法快速调查田间新蚜虫疠霉的流行
Using Modified Chelex Method for Rapid Epizootiological Investigation of Entomophthoralean Fungi (Pandora neoaphidis)
摘要
Abstract
Direct sampling, detection and quantification of entomopathogens can directly reflect the epizootiology of pest in abundance. Pandora neoaphidis, a widespread obligated-aphid pathogen, was investigated in aphid populations. A PCR technology system based on modified Lysis extraction associated with Chelex-100 was developed for epizootiological investigation of entomophthoralean fungi on wheat aphid. Results show that the developed method not only provide a rapid and accurate tool for epizootiological investigation of entomopathogenic fungus, but also have a potential application for rapid and tiny-nucleic acid detection in other fungus.关键词
Chelex/PCR/流行调查Key words
Chelex/PCR/epizootiological investigation分类
农业科技引用本文复制引用
陈相波,陈春,沈金慧,刘秉义..利用改良的Chelex方法快速调查田间新蚜虫疠霉的流行[J].中国生物防治学报,2014,(6):731-735,5.基金项目
浙江省自然科学基金杰出青年项目（LR12C03001）；国家自然科学基金 （）
