中国生物防治学报Issue(6)：743-749,7.
不同交配型球孢白僵菌在玉米植株中的定殖及其交配型基因变异比较研究
A Comparision Studies of Different Mating-type Beauveria bassiana Colonization in Corn, Zea mays, and Mating-type Gene Mutation
摘要
Abstract
In this study, colonization efficiency of the two mating-type Beauveria bassiana (MAT1−1−1, mating-type I; MAT1−2−1, mating-type II) in maize was assessed and the mating-type gene sequences of recovered B. bassiana strains were analyzed to reveal the host plants selection rules between the two mating-type strains and the environmental adaptability of different mating-type strains during colonization process. Four inoculation trials were applied as follows:BbOFDH1−5 (mating-type I) (treatment B5);BbDPSD2 (mating-type II) (treatment B2); mating-type I/mating-type II mixed equally (treatment B5+B2) and control (CK), spore concentration for treatment B5, B2 and B5+B2 was 108 spores/mL and zero for control. Results showed that endophytes were detected in treatment B5, B2 and B5+B2 in corn leaves. Detection rates showed treatment B2 with mating-type II strain got a higher detecting rate of 33.00%than that of treatment B5 with mating-type I strain. In treatment B5+B2, the recovered endophytes had a higher rate with mating-type II strains than mating-type I strain with ratio 12:10. Among the mating-type I B. bassiana strains recovered from treatment B5 and B5+B2, MAT1−1−1 gene sequence analysis showed that multiple mutation loci in the non-coding region were observed while only three mutation loci in the coding region during the endophytic colonization process. However, no mutation was found about MAT1−2−1 gene from all the strains recovered from treatment B2 and B5+B2. In conclusion, mating-type II strain BbDPSD2 had a higher colonization efficiency than that of mating-type I strain BbOFDH1−5; corn plants prefered mating-type II strain BbDPSD2 to mating-type I strain BbOFDH1−5 as endophytic fungus; the mating-type gene MAT1−1−1 had a higher mutation rates than that of MAT1−2−1 significantly indicated that mating-type I strain had stronger environmental adaptability than that of mating-type II strain.关键词
球孢白僵菌/玉米植株/内生定殖/交配型基因变异Key words
Beauveria bassiana/corn plant/endophytic colonization/mating-type gene mutation分类
农业科技引用本文复制引用
李晓慧,汪洋洲,张军,徐文静,张正坤,刘艳微,孟鑫睿,冯树丹,李启云..不同交配型球孢白僵菌在玉米植株中的定殖及其交配型基因变异比较研究[J].中国生物防治学报,2014,(6):743-749,7.基金项目
黑龙江省自然科学基金 （）
