中国实验动物学报Issue(2)：36-39,4.DOI:10.3969/j.issn.1005-4847.2014.02.008
Olig2在 cuprizone 介导的脱髓鞘动物模型中的表达变化
Expression pattern of transcription factor Olig2 in cuprizone-induced mouse model of acute demyelination
摘要
Abstract
Objective To investigate the expression pattern of transcription factor Olig 2 in cuprizone-induced mouse model of acute demyelination .Methods C57BL/6 mice were fed with 0.2%cuprizone to induce acute demyelina-tion.Immunofluorescence and qRT-PCR were used, and Olig2, MBP and GFAP were detected in the brain tissues of con-trol group and cuprizone-treated groups for 6 weeks and recovery for 2 weeks.Results Severe demyelination occurred in the corpus callosum following 6-weeks exposure to cuprizone , while remyelination was detected in the white matter after the mice were given diet without cuprizone .In the normal mice , Olig2 was expressed in a low level , while the experessions of Olig2 and GFAP were significantly increased , and Olig2 +/GFAP+cells were detected after demyelination .But the expres-sion of MBP was below the normal level with demyelination .After recovery for 2 weeks, the experession of Olig2 was lower, but the experessions of MBP and GFAP were increased .Conclusions Olig2 may play an important role in the glial differ-entiation from neural progenitor cells into active astrocytes , and in the glial scar formation .关键词
多发性硬化/Cuprizone/髓鞘再生/Olig2/小鼠Key words
Multiple sclerosis/Cuprizone/Remyelination/Olig2/Mice分类
生物科学引用本文复制引用
陈丽萍,张静,马顺利,李振飞,张金丽,董梅,单明月,郭力..Olig2在 cuprizone 介导的脱髓鞘动物模型中的表达变化[J].中国实验动物学报,2014,(2):36-39,4.基金项目
河北省医学科学研究课题（项目编号20130506）。 （）
