中国实验动物学报Issue(2)：62-65,4.DOI:10.3969/j.issn.1005-4847.2014.02.014
三种不同冷冻保护剂对小鼠附睾冷冻效果的比较
Comparison of the effect of three cryoprotectants on vitrification-cryopreservation of mouse epididymis
摘要
Abstract
Objective To compare the effect of three cryoprotectants on vitrification-cryopreservation of C57BL/6J mouse epididymis.Method Epididymises from 6-8-week old male C57BL/6J mice (age) were cryopreserved using DMSO, PROH and R18S3 cryoprotectant solution and thawed , respectively.The morphology of sperm from thawed epididy-mis, the rate of post-thawing survival and reproductive capacity were determined to evaluate the freezing efficiency of the three cryoprotectants .Results The sperms from thawed epididymis of the three groups were all well-preserved structural-ly.The survival rate of sperms was 88.17%, 61.17% and 16.83% in the PROH, DMSO and R18S3 cryoprotectant solu-tions, respectively, and there were significant differences between the three cryopreservation groups (P<0.05 for all). The number of pups from the DMSO , PROH and R18S3 groups were 13, 8 and 17 mice after ICSI and embryo implantation , respectively.Conclusions All the three cryoprotectants DMSO , PROH and R18S3 solutions are suitable for vitrification-cryopreservation of C57BL/6J mouse epididymis.But PROH is the preference of these cryoprotectant solutions .关键词
小鼠附睾/冷冻保护剂/冷冻效果Key words
Mouse/Epididymis/Cryoprotectant solution/Freezing efficiency/Vitrification分类
生物科学引用本文复制引用
李淼,郁丽丽,张艺宝,强苏静,刘丽均,徐平..三种不同冷冻保护剂对小鼠附睾冷冻效果的比较[J].中国实验动物学报,2014,(2):62-65,4.基金项目
上海市科研计划项目（111409022700）；国家科技支撑计划课题（2013BAK11B02）。 （）
