中国实验动物学报Issue(3)：44-49,6.DOI:10.3969/j.issn.1005-4847.2014.03.000
PCR-测序法对啮齿类动物汉坦病毒的检测和基因分型
PCR sequencing for detection and gene typing of hantavirus isolated from rodents
摘要
Abstract
Objective To evaluate the application value of PCR-sequencing in clinical detection of hantavirus in rodents .Methods Based on 7 subtypes and 24 strains of representative hantavirus strains downloaded from Genbank , the virus S gene fragments were used for primer design and neighbor joining method was applied for phylogenetic analysis . Thereafter, we identified hantavirus strains isolated from wild rodents in recent years in Zhejiang Province by this method . Results The 24 analyzed strains were divided into 5 regions in the phylogenetic tree .Four of them with topology structure were more stable .Eleven strains of the virus were amplified by PCR and sequenced , and the results showed that the prim-ers were with high sensitivity and specificity .Three HTN strains and 1 strain of serotype SEO were distinguished from 9 strains of unknown strains isolated in Zhejiang Province .We also found that 5 strains of hantavirus belonging to two un-known serotypes .Discussion Our results suggest that the PCR-sequencing method proposed in this study can be used for clinical detection of hantavirus .关键词
汉坦病毒/啮齿动物/测序/分型Key words
Hantavirus/Rodent animals/Sequencing/Gene typing分类
生物科学引用本文复制引用
戴方伟,宋晓明,卢领群,周莎桑,萨晓婴,吕宇,应华忠..PCR-测序法对啮齿类动物汉坦病毒的检测和基因分型[J].中国实验动物学报,2014,(3):44-49,6.基金项目
科技部“十二五”科技支撑计划项目子项目（2013BAK11B01-41），浙江省医药卫生科技计划项目（2011RCB001），浙江省科技计划项目（2012C37097、2011F30009、2013F10071）。 （）
