中国实验动物学报Issue(4)：12-15,4.DOI:10.3969/j.issn.1005-4847.2014.04.003
一次性静脉注射高剂量链脲佐菌素建立1型糖尿病小型猪模型
Establishment of a miniature pig model of type 1 diabetes by single intravenous injection of high dose streptozotocin
摘要
Abstract
Objective To explore the feasibility of establishing mini-pig model of type 1 diabetes by single intrave-nous injection of high dose streptozotocin .Methods Eight male Zhonghua mini-pigs (22.6 ±1.8 kg) were administrated with high-dose streptozotocin (150 mg/kg) into the ear vein .Before and 10, 30, 90 minutes, 1, 3 and 7 days after adminis-tration of streptozotocin , blood samples were obtained respectively , and used to dynamically monitor the fasting blood-glucose. C peptide and insulin levels were evaluated by IVGTT test .Results Since 24 hours after STZ administration , the fasting blood-glucose level was increased significantly compared with that of pre-administration and maintained at 16.7-20.6 mmol/L, while the C peptide and insulin levels were decreased significantly .IVGTT results showed that blood sugar levels at 1 h af-ter intravenous injection of 50%glucose were much higher than 11.1 mmol/L and failed to restore to fasting glucose levels until 2 h, insulin and c-peptide did no response after injection of glucose , always kept at a trace level .Conclusion A sin-gle high-dose streptozotocin injection can be used to establish a mini-pig model of type 1 diabetes successfully .关键词
1型糖尿病,小型猪,链脲佐菌素/血糖Key words
Type 1 diabetes/Mini-pig/Streptozotocin/Blood glucose分类
生物科学引用本文复制引用
崔永春,李凯,张宏,刘晓鹏,袁卫民,李巨波,吴爱丽,唐跃..一次性静脉注射高剂量链脲佐菌素建立1型糖尿病小型猪模型[J].中国实验动物学报,2014,(4):12-15,4.基金项目
北京市科委科技计划“常见心脑血管缺血性疾病模型研发公共平台建设”（Z101107052210004）基金；协和青年基金“糖尿病小型猪急性心肌缺血代谢组学研究（2012-XHQN04）支持。 （）
