中国实验动物学报Issue(4)：31-36,6.DOI:10.3969/j.issn.1005-4847.2014.04.008
孕激素暴露致食蚊鱼形态雄性化的生物学效应
Progesterone induces morphological masculinization in immature female mosquitofish (Gambusia affinis)
摘要
Abstract
Objective The aim of this study was to investigate the masculinizing effects of progesterone ( PRO) exposure at different concentrations on the morphology of female mosquitofish (Gambusia affinis).Methods Immature female G.affinis individuals were put into static water with 0.5, 5, 50 and 500 nmol/L progesterone (n=56), respective-ly.In addition, control group and parallel groups were set up .After 42-day-long exposure, we measured four main indexes of the G.affinis:the body length, body weight, morphological changes in the anal fin 3rd fin and the 14, 15 and 16 verte-bral ribs.We also observed the state of ovarian development .Results The body lengths (BL) of experimental groups ex-posed at concentration of 50 and 500 nmol/L progesterone for 42 d showed significant differences , respectively ( P<0.01 for both), when compared with those of the control group .The body weights (BW) of experimental groups exposed to pro-gesterone at all concentrations were significantly or very significantly decreased (P<0.05 or P<0.01).Only the proges-terone at 5 nmol/L concentration showed significant effect on the body health index (CF) (P<0.05).The section number (FJ) of the anal fin 3rd fin, the section length (FL)and the widest portion width (FW)in the experimental groups had sig-nificant difference (P<0.05), respectively, showing significant changes in morphology after the exposure to 500 nmol/L progesterone .However , there was no significant difference between the values resulted by progesterone in other concentra -tions (P>0.05).When exposed to 500 nmol/L PRO, the L, D and L:D values of the 14th, 15th and 16th vertebral ribs of the female G.affinis had very significant difference ( P<0.01 for all ) , respectively , indicating significant changes of morphological masculinization .Histological examination revealed that the developmental status of the ovaries of G.affinis in the experimental groups were to different degrees inhibited , and the nuclei appeared swelling in stage II and III oocytes . Conclusions The results of this study indicate apparent masculinizing effects of progesterone on immature female mosquit -ofish.关键词
食蚊鱼/孕激素/雄性化/组织形态学Key words
Mosquitofish ( Gambusia affinis)/Progestatione/Masculinization/Morphology分类
医药卫生引用本文复制引用
范俊杰,黎紫兰,徐少群,何文婷,黎美媚,闫月明,方展强..孕激素暴露致食蚊鱼形态雄性化的生物学效应[J].中国实验动物学报,2014,(4):31-36,6.基金项目
华南师范大学第8期大学生课外科研项目；广东高校城市水环境生态治理与修复工程技术研究中心建设项目（2012gczxA004）。 （）
