中国实验动物学报Issue(4)：41-46,6.DOI:10.3969/j.issn.1005-4847.2014.04.010
电针对局灶脑缺血／再灌注模型大鼠缺血海马区血管再生的影响及其机制
Effect of electroacupuncture on angiogenesis in rat ischemic hippocampus after focal cerebral ischemia/reperfusion and its mechanism
摘要
Abstract
Objective To explore the effect of electroacupuncture on CD 34 +VEGFR2 +endothelial progenitor cell (EPC)-derived vessels and stromal cell-derived factor-1α(SDF-1α)/CXCR4, and study its mechanism of promoting an-giogenesis in hippocampus after focal cerebral ischemia /reperfusion .Methods A total of 180 healthy male adult Sprague Dawley (SD) rats were randomly divided into sham operation (sham) group, model (I/R) group, electroacupuncture (I/RE) group, I/RE plus AMD3100 (A specific antagonist of CXCR4) group (I/REA) and AMD3100 (I/RA) group. The rats received filament occlusion of the right middle cerebral artery for 2 hours followed by reperfusion .Electroacupunc-ture was applied to “Baihui” (GV20)/“Siguan” (Hegu LI 4/Taichong LR 3) acupoints for 30 min, once a day.The mR-NA expression of SDF-1αand CXCR4 were detected by reverse transcription-polymerase chain reaction ( RT-PCR) .Double immunofluorescence was used to stain CD 34 +VEGFR2 +EPC-derived vessels.Results Compared with the sham group, the mRNA expressions of SDF-1αand CXCR4 were significantly upregulated in I/R and I/RE group ( P<0.05 ) , but that in I/RE group was more significantly increased than I/R group(P<0.05).In addition, the mRNA expression of SDF-1αand CXCR4 were highly increased on day 1 in the I/REA group than that of I/RE group, but decreased than that of I/RE group on day 7 after reperfusion (P<0.01).CD34 +VEGFR2 +EPCs-derived vessels were obviously increased on 3d and 7d in the I/RE group compared with that of the I/R group, and significantly decreased on 7d in the I/REA group compared with that of the I/RE group ( P<0.01) .Conclusions Electroacupuncture can effectively promote an-giogenesis through upregulating the expression of SDF-1αand CXCR4 in rat ischemic hippocampus after focal cerebral is-chemia/reperfusion.关键词
局灶脑缺血/再灌注/SDF-1α/血管再生/电针/大鼠Key words
Focal cerebral ischemia /reperfusion/SDF-1α/Angiogenesis/Electroacupuncture/Rat分类
医药卫生引用本文复制引用
谢宸宸,罗勇,高祥,庞月珊,李满,汶海琪,陈瑞芳..电针对局灶脑缺血／再灌注模型大鼠缺血海马区血管再生的影响及其机制[J].中国实验动物学报,2014,(4):41-46,6.基金项目
教育部“高等学校博士学科点专项科研基金”联合资助课题（20095503110001）；重庆市卫生局中医药科研重点项目（ZY20131027）；重庆市卫生局中医药科研项目（渝中医2005-B-24）。 （）
