中国实验动物学报Issue(4)：47-51,5.DOI:10.3969/j.issn.1005-4847.2014.04.011
钙蛋白酶抑制蛋白转基因小鼠的构建和鉴定
Establishment and identification of calpastatin transgenic mouse models
摘要
Abstract
Objective To establish an animal model of calpastatin ( CAST) transgenic mice by inserting the full hu-man CAST into the genome of C57BL/6J mice.Methods Recombinant transgenic vector pRP .EX3d-EF1A-CAST-IRES-eGFP was constructed by Gateway technology .It was injected into the fertilized eggs from C 57BL/6J mice.The injected eggs were transplanted into the oviduct of pseudopregnant mice .Tail DNA PCR screening was performed to identify the positive founder mice.The expressions of CAST mRNA and protein in tissues of the transgenic mice were detected by RT -PCR and Western blotting.Results Ninty eggs were transplanted into the oviducts of 3 recipients.The transplantation success rate was 100%.23 viable offsprings were born from the recipients .Tail DNA PCR screening showed that two of the offsprings were positive transgenic mice .The positive rate of transgenic mice was 9%.RT-PCR assay revealed that CAST mRNA ex-pressions were present in the heart , liver, kidney, lung, spleen, brain and skeletal muscle of the transgenic mice .Addition-ally, the CAST protein expression was significantly increased in the transgenic mice .Conclusion CAST transgenic mice have been successfully established and provide a good animal model support for further studies on the CAST function .关键词
钙蛋白酶抑制蛋白/转基因/小鼠,模型/显微操作法Key words
Calpastatin/Transgenic/Mice, models/Microinjection分类
生物科学引用本文复制引用
叶廷巧,马双陶,李丹,郑曦,王强,苏立男,张彦,杨芸,杨永健..钙蛋白酶抑制蛋白转基因小鼠的构建和鉴定[J].中国实验动物学报,2014,(4):47-51,5.基金项目
成都军区总医院科研项目（2011YG-A11）；2013全军医学科技青年培育项目（13QNP058）。 （）
