中国实验动物学报Issue(4)：56-59,4.DOI:10.3969/j.issn.1005-4847.2014.04.013
移植部位对小鼠睾丸移植效果的影响
Effect of different transplantation sites on the outcome of testicular grafts in mice
摘要
Abstract
Objective To compare the effects of different transplantation sites on the outcome of testicular grafts in mice, and to provide a basis for development and application of this technique in relevant research .Method 5-day old and 4-week old SPF male C57BL/6J mice were used in this study .Three groups of testicular transplantation , i.e.dorsal subcutaneous transplantation ( 5 mice, 40 testes ) , transplantation inside the testicular tunica albuginea ( 6 mice, 12 testes), and were subrenal capsule transplantation (10 mice,15 testes) groups were set up for evaluating the effect of transplantation site on the outcomes in mice .Sham operation (4 mice) and castration (4 mice) groups were also used in this study.The mice were sacrificed at 8 weeks after transplantation and the transplanted testes were collected for analysis of their weight, transplant survival, weight gain, and germ cell differentiation.Results There were significant differences of the testicular germ cell differentiation in different transplantation groups .The germ cell differentiation was best in the in-tra-tunica albuginea transplantation group , and were similar to that in the sham operation group .The germ cell differentia-tion rate was 100%in the intra-tunica albuginea transplantation group , 29.2% in the subcutaneous transplantation group and 0%in the subrenal capsule transplantation group .Conclusions Transplantation beneath the testicular tunica albug-inea is the most favorable site for germ cell differentiation , and dorsal subcutaneous transplantation is an alternative choice . Subrenal capsule transplantation is not appropriate for preservation of male reproductive organs in mice .关键词
小鼠/睾丸移植/移植部位/生殖细胞分化Key words
Mice/Testicular transplantation/Transplantation site/Germ cell differentiation分类
生物科学引用本文复制引用
张艺宝,李淼,郁丽丽,强苏静,刘丽均,徐平,芮荣..移植部位对小鼠睾丸移植效果的影响[J].中国实验动物学报,2014,(4):56-59,4.基金项目
国家科技支撑计划课题（2013BAK11B02），上海市科学技术委员会科研计划项目（11140902700）。 （）
