中国实验动物学报Issue(5)：7-12,6.DOI:10.3969/j.issn.1005-4847.2014.05.002
探索 Sertoli 细胞对去除精原细胞的动态反应
Exploration of the dynamic responses of Sertoli cells to depletion of spermatogonial stem cells in mouse testis
摘要
Abstract
Objective To explore the dynamic responses of Sertoli cells to depletion of spermatogonial stem cells by busulfan.Methods After intraperitoneal injection of 15, 30 or 44 mg/kg busulfan to mice, the spermatogenesis and the expression of GDNF, PLZF, Nanog and GFRɑ1 mRNA were assessed by real-time quantitative PCR at 5 and 28 days after the busulfan treatment.Results Glial cell line-derived neutrophic factor ( GDNF ) was significantly increased and showed a dose-dependent trend at 5 days after busulfan treatment, but no significant difference was seen in the expression of promyelocytic leukemia zinc finger(PLZF) and GDNF family receptorα-1(GFRα1).The testicular histology also appeared no significant difference at 5 days after busulfan treatment.At 28 days after busulfan treatment, the relative expression lev-els of GDNF, PLZF, Nanog and GFRɑ1 mRNA were drastically increased.Morphological observation showed that spermat-ogenesis damages became even more severe as the busulfan dose increased.Conclusions Sertoli cell response to the de-pletion of spermatogonia occurs as early as the fifth day after busulfan treatment.Production of GDNF in Sertoli cells shows a compensatory increase, which may stimulate spermatogonial stem cells to accelerate their self-renewal, reflected by the enhancing expression of Nanog and PLZF, and ultimately promote the restoration of spermatogenesis.关键词
白消安/精原干细胞/Sertoli细胞/睾丸/小鼠Key words
Busulfan/Spermatogonial stem cell/Sertoli cell/Testis/Mouse分类
生物科学引用本文复制引用
安娜,袁丽芳,王善林,李宗悦,王峰,朱宝长..探索 Sertoli 细胞对去除精原细胞的动态反应[J].中国实验动物学报,2014,(5):7-12,6.基金项目
国家自然科学基金（ No．30870934）。 （）
