中国实验动物学报Issue(5)：22-26,31,6.DOI:10.3969/j.issn.1005-4847.2014.05.005
脂多糖和石墨粉诱导小鼠急性肺损伤病理形态学比较
Pathological comparison of lipopolysaccharide-and graphite particle-induced acute lung injury
摘要
Abstract
Objective To compare the differences of lung pathological changes of acute lung injury in mice in-duced by lipopolysaccharide ( LPS) and graphite particles, and to explore the possible mechanisms of acute lung injury in-duced by fine particles of different origins.Methods 140 male specific-pathogen-free Kunming mice weighing 18-20 g were randomly divided into 7 experimental groups, in addition to the normal control group.The experimental groups were treated by intratracheal instillation of LPS solution or graphite powder suspension in different doses, respectively, to induce acute lung injury in the mice.The mortality of the mice was observed, and pathological changes of the lung tissues were ex-amined by light and transmission electron microscopy.Western blot was used to detect the protein expression of neutrophil elastase ( NE) in lung tissues , and real-time quantitative PCR was used to detect mRNA expression of monocyte chemotac-tic protein-1 ( MCP-1) in the lung tissue .Results Compared with the normal control group, some pathological changes were observed in the lung tissues of the groups L ( LPS) and G ( graphite) .There were numerous macrophages in the lung tissues in the group G mice, and exudate, mainly neutrophils, in the lung tissues of the group L.The NE protein expres-sion in the lung tissue was significantly higher than that of the normal control group ( P<0.05) , and there was also a sig-nificant difference between the groups L and G (P<0.05).The MCP-1 mRNA expression in lung tissues was higher in the control group (P<0.01), and there was also a significant difference between the groups L and G (P<0.01).Conclu-sions Diverse types of particulate matters induce different pathological changes in the lungs, therefore the mechanism may also be different in the inflammatory responses.It means that the lung injuries caused by fine particles of mixed composition may have complex mechanisms.关键词
急性肺损伤/脂多糖/石墨粉/作用机制/病理学Key words
Acute lung injury/Lipopolysaccharides, LPS/Graphite powder/Mechanism/Pathology/Mice分类
生物科学引用本文复制引用
崔雯雯,张彦芬,秘尧,金鑫,刘克剑,王宏涛..脂多糖和石墨粉诱导小鼠急性肺损伤病理形态学比较[J].中国实验动物学报,2014,(5):22-26,31,6.基金项目
国家科技部“十二五重大新药创制”项目抗流感中药连花清瘟胶囊生产技术改造研究（编号2011ZX09201-201-27）。 （）
