中国实验动物学报Issue(5)：32-34,3.DOI:10.3969/j.issn.1005-4847.2014.05.007
肺癌转移瘤动物模型的建立
Establishment of a nude mouse model of pulmonary adenocarcinoma metastasis
摘要
Abstract
Objective To establish an optimal animal model of pulmonary metastasis of human lung adenocarcino-ma, to serve further investigation of mechanism of lung adenocarcinoma metastasis.Methods Eleven nude mice aged 4-6 weeks were used in this study.Suspension of human lung adnocarcinoma A549 cells (0.1 mL, 107 cells/mL) was injec-ted into the tail vein in nude mice.From four weeks after inoculation, two nude mice were killed each time at 4, 5, 6 weeks after the tumor cell injection at random for examination.The remaining 3 mice were killed at the end of the experi-ment.At autopsy, the lung, brain, liver, kidney and other organs were removed, fixed in neutral buffered formalin and embedded in paraffin.Sections were cut and stained with hematoxylin-eosin, and examined by histopathology.The number of metastatic foci was counted.Results No mouse died after tumor cell inoculation.Serially euthanized mice revealed evi-dence of gradually increasing pulmonary metastases in the mice:No metastasis was found before 4 weeks after tumor cell in-oculation, the first histological metastases appeared at 5 weeks, gross metastatic foci were observed at 6 weeks, widely spread metastatic foci were observed at 7 weeks, and the remain 3 mice developed cachesia at 11, 13, and 14 weeks after tumor cell inoculation.Mediastinal lymph node metastases were found in the nude mice by 11 weeks after tumor cell inocu-lation.Conclusions We have successfully established a nude mouse model of pulmonary metastasis by injecting human lung adenocarcinoma A549 cells into the tail vein.关键词
肺腺癌/肿瘤转移/动物模型/裸鼠Key words
Lung adnocarcinoma/Neoplasm metastasis/Animal model/Nude mice分类
生物科学引用本文复制引用
陈愉生,林小芬,李鸿茹,林献,林明,许能銮..肺癌转移瘤动物模型的建立[J].中国实验动物学报,2014,(5):32-34,3.基金项目
福建省科学自然资金，livin基因致肺癌侵袭及作用机制研究（2011J01130）。 （）
