中国实验动物学报Issue(5)：35-38,4.DOI:10.3969/j.issn.1005-4847.2014.05.008
抑制T细胞增殖的抗CD45单克隆抗体的建立
Establishment of an anti-CD45 monoclonal antibody that inhibits T-cell proliferation
摘要
Abstract
Objective To identify molecules that modulate T-cell functions and serve the studies on T-cell media-ted autoimmune diseases.Methods Bone marrow-derived dentritic cells were collected from BALB/c mice to immunize Wistar rats, and to establish many hybridoma cell lines.Many hybridoma cell lines which could modulate T-cell functions were obtained.One of the cell lines, most actively inhibiting T-cell proliferation, was further studied.Results The anti-CD45 mAb recognized CD45 and significantly suppressed T-cell proliferation in proliferation assays.Conclusions Our re-sults indicate that the anti-CD45 mAb can effectively suppress T-cell proliferation, and is promising to be used in the pre-vention and treatment of T-cell mediated autoimmune diseases in the future.关键词
CD45/单克隆抗体/抑制/T细胞增殖/树突状细胞/小鼠/大鼠Key words
CD45/Monoclonal antibody/Inhibition/T-cell proliferation/Mouse/Rat/Dendritic cells分类
生物科学引用本文复制引用
苏泽红,李亚林,谭翔文,余坚,刘鑫,袁月亲,练高建..抑制T细胞增殖的抗CD45单克隆抗体的建立[J].中国实验动物学报,2014,(5):35-38,4.基金项目
湖南省科技厅项目（2013TT2007，2014TT2012），湖南省教育厅优秀青年项目（13B102），湖南省自然基金（13JJ3081）资助。 （）
