中国实验动物学报Issue(5)：53-58,6.DOI:10.3969/j.issn.1005-4847.2014.05.012
不同干预方法对去卵巢骨质疏松大鼠子宫GSK-3β蛋白表达的影响
Effects of different interventions on the GSK-3βprotein expression in uterus of ovariectomized osteoporotic rats
摘要
Abstract
Objective The aim of this study was to observe the effects of whole-body vertical vibration, treadmill exercise, genistein administration and estrogen injection on the uterus weight index and uterus histology as well as the ex-pression of glycogen synthase kinase ( GSK-3β) protein in the uterus of ovariectomized osteoporotic rats.Methods Sev-enty-two healthy 3-month old female SD rats were randomly divided into two groups by body weight:sham-operation group (Sham) and ovariectomized group (OVX).At 10 weeks after OVX operation, the OVX rats were randomly divided into the following five groups by body weight: OVX group, whole-body vertical vibration group (WBVV), treadmill exercise group ( TX) , genistein group ( G) and 17β-estrogen group ( E2 ) .Then they were treated with different methods according to the experiment design.At the end of experiment, the uterus weight was measured with an electronic balance.The uterus histology was observed with HE staining and the expressions of GSK-3βand P-GSK-3βproteins were detected by Western blot.Results Apart from E2 treatment, all the other three treatments did not increase the uterus weight and the thickness of endometrium compared with that in the OVX rats.Apart from genistein treatment, all the other three treatments increased the ratio of protein expression of P-GSK-3βto GSK-3βcompared with that in the OVX rats.Conclusions Both whole-body vertical vibration and treadmill exercise can stimulate the phosphorylation of GSK-3βin the uterus of OVX osteoporotic rats.However, genistein has no such stimulation effect.关键词
去卵巢大鼠/全身振动/跑台运动/金雀异黄酮/子宫Key words
Ovariectomized rats/Whole-body vertical vibration/Treadmill exercise/Genistein/Uterus分类
生物科学引用本文复制引用
郭盖,卜淑敏,韩天雨..不同干预方法对去卵巢骨质疏松大鼠子宫GSK-3β蛋白表达的影响[J].中国实验动物学报,2014,(5):53-58,6.基金项目
北京市教委市属高等院校长城学者项目（ CIT＆TCD20130337）；北京市教委科技创新平台项目（ No．PXM2012-014206-000051）。 （）
