中国实验动物学报Issue(5)：59-62,4.DOI:10.3969/j.issn.1005-4847.2014.05.013
FH／W jd 大鼠酒精性肝损伤模型探讨
Exploration of a FH/Wjd rat model of alcoholic liver disease
摘要
Abstract
Objective To investigate the FH/Wjd rat model of alcoholic liver disease.Methods Thirty-six 16-18 week old SPF grade FH/Wjd rats (male:female=1:1) were used in this study.The rats were divided into two groups randomly by body weight:water intake group and alcohol intake group.The rats took water or alcohol freely.16 weeks lat-er, ALT, AST, TBIL, TG, CHO in the serum and TG, GSH in the liver homogenate were detected.The expression of PPARαprotein in the liver tissue was detected by Western blot.The apoptosis rate of liver cells was assessed by flow cy-tometry.The pathological changes of liver tissue were examined using HE staining.Results Compared with the water in-take group, the serum TBIL and TG were significantly increased in rats of both sexes of the alcohol intake group, moreover, ALT and CHO of the female rats in the alcohol intake group were significantly decreased.TG in the liver homogenate in-creased obviously, while GSH in the liver homogenate showed a decreasing tendency.Hepatocyte apoptosis in rats of both sexes in the alcohol intake group showed an increasing tendency.The PPARαprotein expression was up-regulated obvious-ly, and the main pathological change in the liver tissue was microvesicular fatty degeneration.Conclusion Spontaneous long-term alcohol intake can induce liver injuries in FH/Wjd rats.关键词
FH/W jd大鼠/酒精/肝脏损伤/PPARα蛋白Key words
FH/Wjd rat/PPARα/Alcoholic liver disease分类
生物科学引用本文复制引用
张彦芬,秘尧,何奇龙,王宏涛..FH／W jd 大鼠酒精性肝损伤模型探讨[J].中国实验动物学报,2014,(5):59-62,4.基金项目
太行山道地、大宗药材综合利用技术及产业化开发项目（13042506Z）。 （）
