中国实验动物学报Issue(5):67-70,4.DOI:10.3969/j.issn.1005-4847.2014.05.015
GFP 在绿色荧光裸鼠不同组织中的表达及分析
Distribution of green fluorescent protein (GFP) expression in green fluorescent nude mice
摘要
Abstract
Objective To study whether the green fluorescent protein ( GFP) gene can be successfully expressed in green fluorescent nude mice and the tissue distribution characteristics.Methods Small animal imaging system and RT-PCR assay were used to detect the GFP tissue distribution and fluorescence expression level.Results The GFP can be expressed in multiple tissues in green fluorescent nude mice.A higher expression was observed in the pancreas, heart, brain, and skin.Conclusion Exogenous GFP can be stably expressed and inherited in green fluorescent nude mice, with the highest expression in the pancreas.关键词
绿色荧光蛋白/裸鼠/组织分布Key words
Green fluorescent protein ( GFP)/Nude mice/Tissue distribution分类
生物科学引用本文复制引用
尤金炜,赵志刚,恽时锋,董敏,刘彪,梁磊,田迎,胡文娟,田小芸,方天,周森妹..GFP 在绿色荧光裸鼠不同组织中的表达及分析[J].中国实验动物学报,2014,(5):67-70,4.基金项目
南京军区南京总医院课题(2012052)。 ()