中国实验动物学报Issue(6)：17-21,5.DOI:10.3969/j.issn.1005-4847.2014.06.003
CLCN3／MMTV－PyMT 转基因小鼠杂交群体的建立及鉴定
Establishment and identification of a ClC-3/MMTV-PyMT hybrid mouse strain
摘要
Abstract
Objective To establish a CLCN3/MMTV-PyMT double transgenic mouse model of spontaneous breast tumor with simultaneously overexpressing ClC-3.Method CLCN3 transgenic mice were crossed with MMTV-PyMT spon-taneous mammary tumor model mice.The genotype was determined by PCR.The expression of ClC-3 in tissues was detec-ted by immunofluorescence and Western blot.Results CLCN3 and MMTV-PyMT transgenic mice were bred and CLCN3/MMTV-PyMT hybrid mouse model was successfully established.The ClC-3 expression in CLCN3/MMTV-PyMT hybrid mice was higher than that in the MMTV-PyMT mice, assessed by immunofluorescence and Western blot analysis.Conclu-sions Transgenic mouse models of spontaneous breast cancer with simultaneously overexpressing ClC-3 are successfully es-tablished.The double transgenic mice provide a good animal model for further research of ClC-3 in tumor growth and metas-tasis.关键词
CLCN3/MMTV-PyMT/转基因小鼠/乳腺癌Key words
CLCN3/MMTV-PyMT/Transgenic mice/Breast cancer分类
生物科学引用本文复制引用
邓璐璐,李琴,吴慧,毛建文,徐彬..CLCN3／MMTV－PyMT 转基因小鼠杂交群体的建立及鉴定[J].中国实验动物学报,2014,(6):17-21,5.基金项目
国家自然科学基金项目（编号31371144）。 （）
