中国实验动物学报Issue(6)：49-53,5.DOI:10.3969/j.issn.1005-4847.2014.06.009
中药Ⅰ号方对 APP／PS1双转基因模型小鼠 APP 代谢的影响
Effect of a Chinese traditional prescription PN-1 on the amyloid precursor protein processing in transgenic mouse model of Alzheimer’ s disease
摘要
Abstract
Objective To investigate the effects of a Chinese traditional prescription PN-1 on the amyloid precur-sor protein ( APP) processing in a transgenic mouse model of Alzheimer’ s disease.Methods Eighty 5-month old trans-genic mice were randomly divided into vehicle group, PN-1 low (0.6 g/kg), medium (1.2 g/kg) and high (2.4 g/kg) dose groups.Both the wild-type littermates and model group with distilled water administration were chosen as control groups.Sixteen mice (8 males and 8 females) in each group were given intragastrically PN-1 or distilled water once a day for 4 months.After the drug administration, immunohistochemistry, ELISA and Western blot were performed to measure the products of amyloid precursor protein (APP) and the associated enzymes (ADAM10 and BACE1).Results Com-pared with the control group, β-CTF (C99), α-CTF (C83), sAPPα, sAPPβ, ADAM10 and BACE1 were significantly decreased in the low, medium and high dose groups ( P<0.01 ) .Conclusions PN-1 alleviates cognitive deficits in an Alzheimer’ s disease transgenic mouse model through influencing the processing of APP.关键词
中药Ⅰ号方/阿尔茨海默病/APP分解酶/APP/PS1双转基因AD模型小鼠Key words
Chinese traditional medicine, prescription-1 (PN-1)/Alzheimer’s disease/Amyloid precursor pro-tein, APP/APP processing enzyme/APP/PS1 double transgenic mouse model分类
生物科学引用本文复制引用
隋小龙,秦川,邓巍,梁良,张玲,朱华,徐艳峰,黄澜,徐玉环,韩云林,姚志刚..中药Ⅰ号方对 APP／PS1双转基因模型小鼠 APP 代谢的影响[J].中国实验动物学报,2014,(6):49-53,5.基金项目
国家863课题（编号SS2012AA022613）。 （）
