中国实验动物学报Issue(6)：54-59,74,7.DOI:10.3969/j.issn.1005-4847.2014.06.010
诱发性2型糖尿病小鼠模型与自发性db／db 小鼠特性的比较
Comparison of the characteristics of induced and spontaneous db/db mouse models of type 2 diabetes mellitus
摘要
Abstract
Object To establish an induced mouse model of type 2 diabetes mellitus, and compare it with db/db mouse model of spontaneous type 2 diabetes.To evaluate the two mouse models objectively, and provide an experimental basis for the choice of animal model and its practical application in diabetes studies.Methods A mouse model of induced type 2 diabetes was established by feeding C57BL/6J mice with high-fat and high-sugar diet for four weeks and taking daily intraperitoneal injections of streptozotocin ( STZ) for consecutive 3 days.Four weeks after infection, the gross appearance of kidney and liver of the mice was assessed, glucose tolerance was tested, serum biochemical indices and expression of se-rum IL-2, IL-4, IL-6, IFN-γ, TNF-α, IL-17, and IL-10 were assayed, and were compared with those of the db/db mouse models of spontaneous type 2 diabetes.Results Obvious differences were found in the kidneys and liver gross appearance of the two types of mouse models and the control group.Both the two groups showed significant differences in the blood glu-cose levels at each time point (P<0.05) and low glucose tolerance function, but there were no significant differences in blood glucose levels of the two types of mouse models.Compared with the control group, the serum biochemical indices GLU, GHOL and LDLC of the two types of mouse models were significantly increased (P<0.05).Meanwhile, the blood lipid level of the mouse model of induced type 2 diabetes was higher than that of the db/db mouse models of spontaneous type 2 diabetes.In comparison of immune indices, except IL-2,the serum cytokine levels of the two types of mouse models were significantly higher than those of the control group (P<0.05).Moreover, the serum cytokine levels of db/db mice were higher than those in the mouse models of induced type 2 diabetes, and the IL-6, IFN-γ, TNF-αalso had obvious differences.Conclusions Both the two types of mouse models of type 2 diabetes successfully simulate the human diabetes to some extent, but there are still certain differences according to different etiology of diabetes.We would suggest that peo-ple may take our data as reference and chose appropriate mouse models according to the requirement of their research.关键词
2型糖尿病/诱发性小鼠模型/db/db小鼠/细胞因子Key words
Type 2 diabetes mellitus/Induced mouse model/db/db mice/Cytokines分类
生物科学引用本文复制引用
刘芳,杨华,周文江,周晓辉..诱发性2型糖尿病小鼠模型与自发性db／db 小鼠特性的比较[J].中国实验动物学报,2014,(6):54-59,74,7.基金项目
上海市科委资助项目（11140903001）。 （）
