中国实验动物学报Issue(2)：111-114,4.DOI:10.3969/j.issn.1005-4847.2015.02.001
猪同种异体原位左肺移植模型的建立
A porcine model of orthotopic left lung transplantation
摘要
Abstract
Objective To establish a porcine allogeneic left lung orthotopic transplantation model to closely simu-late human lung transplantation.Methods Twelve Huanjiang mini-pigs were used as donors and 12 Bama mini-pigs as recipients.The left lung orthotopic transplantation was completed by the left fourth intercostal thoracotomy.At 1 h, 2 h, 4 h, 6 h, 12 h after transplantation, the left and right pulmonary artery pressure were measured, the left and right pulmonary vein blood gas was analyzed, and samples of the left and right lung tissues were taken to determine the water content and for pathological examination.Results All animals survived, and the transplanted pulmonary vein blood PaO2/FiO2 and PAP were rised along with the prolonged postoperative time, compared with those of the recipient normal lung showing a signifi-cant difference (P<0.05).With the pass of time, there were increasing edema, inflammatory cell infiltration, RBC ooze, thickening of alveolar wall in the transplanted lung tissue, and some alveolar lumen occlusion and lung tissue consolidation. The water content of the transplanted lung tissue was increased significantly compared with that in the recipient lung tissue ( P<0.05 ) .Conclusions The established method in this study provides an ideal animal model for research on lung transplantation ischemia-reperfusion injury and immune rejection mechanism.关键词
肺移植/猪/动物模型Key words
Lung transplantation/Porcine model/Animal model分类
生物科学引用本文复制引用
曹浩,周文勇,康乐,占贞贞,王竹林,汪波,丁海燕,范慧敏,刘中民..猪同种异体原位左肺移植模型的建立[J].中国实验动物学报,2015,(2):111-114,4.基金项目
浦东新区卫生系统领先人才培养（PWR12011-01）；上海市重点专科心脏外科（ZK2012A27）；上海市浦东新区卫生系统重点学科群（ Grant No．PWZxq2014-01）；教育部高等学校博士学科点专项科研基金（20130072120062）；上海市浦东新区卫生、计生科技项目（PW2013A-2）；上海市青年科技启明星计划（13QA1403100）；同济大学青年优秀人才培养计划（2012KJ043）；上海市东方医院启航计划（DFQH-M4）。 （）
