中国实验动物学报Issue(2)：119-123,5.DOI:10.3969/j.issn.1005-4847.2015.02.003
大鼠腹腔内工作型心脏移植模型的改进
Experience and improvement in establishing an abdominal working heart transplantation model in rats
摘要
Abstract
Objective To modify the techniques for establishment of an abdominal working heart transplantation model in rats and to sum up the key factors to success.Methods A total of 180 12-week old Brown Norway rats ( donor) and Lewis rats ( recipient) were used in this study:50 BN rats and 50 Lewis rats for pilot experiment, and 40 BN rats and 50 Lewis rats for the formal experiment.The rat model of working heart heterotopic transplantation was adopted and estab-lished by Wiedemann’ s mode.We transplanted the heart from BN rats to Lewis rats and analyzed the survival rate, causes of death and histological changes of the heart ( HE staining) in this experiment.Results After exercise and modification, the survival rate was increased to 77.5%, and the mean total duration of operation was 71 ±11 min, and the mean ische-mic time of the donor hearts was 34 ±5 min.Histological examination ( HE staining) of the cardiac allograft showed a mild inflammatory cell infiltration in the graft at 24 h after transplantation, indicating that the model was reliable.Conclusions A variety of factors may affect the final operation success rate in the establishment of this heart transplantation model.A-mong them, the major affecting factors include: healthy animals, donor heart protection, rapid and effective vascular su-ture, and postoperative animal management.关键词
心脏移植/工作型心脏/动物模型/免疫排斥/大鼠Key words
Working heart transplantation/Rat/Animal model/Surgery/Immunological rejection分类
生物科学引用本文复制引用
王竹林,曹浩,占贞贞,范慧敏..大鼠腹腔内工作型心脏移植模型的改进[J].中国实验动物学报,2015,(2):119-123,5.基金项目
上海领军人才（2012-053）；上海市浦东新区卫生系统领先人才（ Grant No．PWR12011-01）；上海市医学重点专科心脏外科（ZK2012A27）；上海市浦东新区卫生系统重点学科群（ Grant No．PWZxq2014-01）；教育部高等学校博士学科点专项科研基金（20130072120062）；上海市青年科技启明星计划（13QA1403100）；上海市浦东新区卫生、计生科技项目（PW2013A-2）；上海市东方医院启航计划（ DFQH-M4）。 （）
