中国实验动物学报Issue(2)：132-138,7.DOI:10.3969/j.issn.1005-4847.2015.02.006
缬沙坦对糖尿病大鼠肾脏中内质网应激及炎症反应的抑制作用
Inhibitory effect of valsartan on endoplasmic reticulum stress and inflammation in the diabetic rat kidney
摘要
Abstract
Objective To study the role of endoplasmic reticulum stress and related inflammation in the kidneys of rats with diabetic nephropathy and the effect of valsartan on these lesions.Methods The diabetic rat model was induced by intraperitoneal injection of streptozotocin.Thirty-four healthy male SD rats were randomly divided into normal control group (n=10), diabetic group (n=12), and valsartan group (n=12).Valsartan (10 mg/kg) was administered daily by gavage from the next day of the diabetes induction for 6 weeks.The expression and distribution of ERS-related proteins P-IRE1α, P-JNK, and MCP-1 were examined by immunohistochemistry and Western blot.Real-time fluorescence quantita-tive PCR was used to detect the mRNA expressions of IRE1α, JNK and MCP-1.The 24-hour urine protein excretion, Scr, and BUN were checked.Results Compared with the control group, infiltration of inflammatory cells was aggravated in the kidneys of DM+V group, the expressions of P-IRE1α,IRE1α,P-JNK,MCP-1 were significantly increased, and the levels of IRE1mRNA and MCP-1mRNA increased compared with the DM group, infiltration of inflammation cells was alleviated in the kidney of DM+V group, the protein expressions of P-IRE1α,IRE1α,P-JNK,MCP-1 were significantly reduced, the levels of IRE1mRNA and MCP-1mRNA were reduced.While there was no significant difference in the expression of JNK mRNA and protions among the three groups.Conclusions ERS and related inflammation are activated in the kidney of di-abetic rats.Inhibition of the IRE1/JNK/MCP-1 pathway of ERS and related inflammation might be responsible for the pro-tective effects of valsartan on the kidneys of diabetic rats.关键词
内质网应激/炎症反应/糖尿病肾病/缬沙坦Key words
Endoplasmic reticulum stress/Inflammation/Diabetic nephropathy/Valsartan分类
生物科学引用本文复制引用
陈凯,张承英,李建民,张建荣..缬沙坦对糖尿病大鼠肾脏中内质网应激及炎症反应的抑制作用[J].中国实验动物学报,2015,(2):132-138,7.基金项目
国家自然基金面上资助项目（No．81273706）。 （）
