中国实验动物学报Issue(2)：139-142,4.DOI:10.3969/j.issn.1005-4847.2015.02.007
IL-1、IL-6、TNF-α及 IFN-γ在脾肾阳虚型溃疡性结肠炎模型大鼠血清及组织中的表达
Expression and significance of IL-1, IL-6, TNF-αand IFN-γin serum and colon tissue in the rat models of ulcerative colitis with spleen and kidney yang deficiency
摘要
Abstract
Objective To detect the levels of IL-1, IL-6,TNF-αand IFN-γin serum and colon tissue of rat mod-els of ulcerative colitis with spleen and kidney Yang deficiency, and to explore their roles in the pathogenesis of ulcerative colitis ( UC) .Methods The rat model of ulcerative colitis with Yang deficiency of spleen and kidney was induced by perfusion of rhubarb decoction plus intramuscular injection of hydrocortisone and combined with TNBS (2,4,6-trinitro-benzenesulfonic acid) and ethanol enema.Sixty SPF wistar rats ( body weight 180 ±10 g, male:female=1:1) were ran-domly divided into blank control group, UC model with spleen kidney Yang deficiency for 7 days, 14 days and 21 days groups, respectively.The levels of IL-1, IL-6, TNF-αand IFN-γin serum and colon tissue were detected by ELISA.Re-sults Compared with the blank group, the levels of IL-1, IL-6, TNF-αand IFN-γin serum and colon tissue of rat UC model group with spleen kidney Yang deficiency were greatly increased (P<0.05), especially evidently increased in the model group at 21 days.Conclusions The pro-inflammatory cytokines IL-1, IL-6, TNF-αand IFN-γplay an important role in the pathogenesis of ulcerative colitis with syndrome of spleen and kidney Yang deficiency.关键词
溃疡性结肠炎/脾肾阳虚/白细胞介素/肿瘤坏死因子-α/干扰素-γ/大鼠Key words
Ulcerative colitis/Yang deficiency of spleen and kidney/Interleukin/Tumor necrosis factor alpha/Interferon gamma/Enzyme linked immunosorbent assay/Rat分类
生物科学引用本文复制引用
殷银霞,许雅清,李海龙,邱家权,程小丽,明海霞,梁永林,吴玉泓..IL-1、IL-6、TNF-α及 IFN-γ在脾肾阳虚型溃疡性结肠炎模型大鼠血清及组织中的表达[J].中国实验动物学报,2015,(2):139-142,4.基金项目
国家自然科学基金项目（81260520）。 （）
