中国实验动物学报Issue(3)：291-296,6.DOI:10.3969/j.issn.1005-4847.2015.03.013
电针介导 eNOS 动员内源性 EPCs 促MCAO／R 大鼠脑内血管再生
Electroacupuncture promotes revascularization via eNOS mobilizing EPCs in the middle cerebral artery occlusion/reperfusion rat
摘要
Abstract
Objective To explore the effect and mechanism of electroacupuncture on eNOS in mobilizing endothe -lial progenitor cells (EPCs) in rat bone marrow and peripheral blood to promote revascularization in focal cerebral ischemi -a/reperfusion rat.Methods A total of 100 healthy male adult Sprague-Dawley (SD) rats were randomly divided into nor-mal group ( N) , model group ( I/R) , electroacupuncture group ( I/RE) and I/RE plus L-NAME ( A specific antagonist of eNOS) group (I/REL), and were further divided into 1 d, 2 d and 7 d subgroups after reperfusion, 10 rats in each group, in addition to the N group .The rats received filament occlusion of the right middle cerebral artery for 1.5 hours followed by reperfusion .“Baihui” ( GV 20)/“Siguan” ( Hegu LI 4/Taichong LR 3) were selected as acupucture points .Flow cytome-ter was used to detect the percentage of EPCs in bone marrow and peripheral blood .The expression of VEGFR2 mRNA was tested by fluorescence quantitative PCR .Immunohistochemical staining was used to detect VEGFR 2 +cells and to stain the CD34 +microvessels.Results Compared with the I/R group, there was a significant up-regulation of the percentage of EPCs in bone marrow and peripheral blood by electroacupuncture (P<0.01,P<0.05), but decreased by the inhibitor of eNOS (P<0.01).Compared with the I/R group, the VEGFR2 +cells, expression of VEGFR2 mRNA and CD34 +mi-crovessels were significantly increased in the I/RE group ( P<0.01 ) , but decreased in the I/REL group ( P<0.01 ) . Conclusions Electroacupuncture can effectively mobilize EPCs to promote the revascularization in focal cerebral ischemia /reperfusion rat .This effect is attenuated by inhibitor of eNOS , suggesting that the activation of eNOS mediated by electroa-cupuncture may be related to mobilizing EPCs to promote the revascularization .关键词
局灶脑缺血/再灌注/EPCs/eNOS/电针/血管再生/脑/大鼠Key words
Focal cerebral ischemia /reperfusion/EPCs/eNOS/Electroacupuncture/Angiogenesis/Brain/Rat分类
医药卫生引用本文复制引用
朱艳含,罗勇,胥虹贝,王盼欣..电针介导 eNOS 动员内源性 EPCs 促MCAO／R 大鼠脑内血管再生[J].中国实验动物学报,2015,(3):291-296,6.基金项目
教育部“高等学校博士学科点专项科研基金”联合资助课题（20095503110001）；重庆市卫生局中医药科研重点（ZY20131027）；重庆市卫生局中医药科研项目（渝中医2005－B－24）。 （）
