中国比较医学杂志Issue(6)：12-15,4.DOI:10.3969/j.issn.1671.7856.2014.006.003
利用超数排卵技术进行优化 C57 BL／6 J小鼠的生物净化体系
Use of optimized superovulation conditions for C57 BL/6 J mouse purification
冯颖 1王芊芊 1陆文昊 2曹敏梁1
作者信息
- 1. 浙江大学实验动物中心，杭州，浙江 310058
- 2. 台州医院生殖中心，台州，浙江 317000
- 折叠
摘要
Abstract
Objective To define the best dose of PMSG/hCG for superovulation and optimize the biological purification system for genetic-modified mice of C57BL/6J background, we injected different dose of PMSG/hCG to promote superovulation, and carried out in vitro fertilization and embryo transplantation.Methods 5-week old female C57BL/6J mice were injected with 4 different doses(5 IU,7.5 IU,10 IU and 15 IU, respectively) of PMSG/hCG.Then the mice were mated with wild type male mice and embryos were obtained by cesarean section.In another approach, oocytes of superovulated mice were fertilized in vitro and then transplanted into surrogate female mice . Results In the cesarean section group, the highest rate of pregnancy was 89.00 ±19.05% in the 5 IU injection group.But the difference compared with other groups was not statistically significant.In the embryo transplantation group, the average number of oocytes from 10 IU injection group was 30.33 ±0.89, and the average number of 2-cell embryo was 23.78 ±0.19, significantly higher than those in the other three groups.Conclusions Administration of 5 IU PMSG/hCG will improve mating efficiency in cesarean section-based purification of C57BL/6J mice.10 IU PMSG/hCG is the best dose for superovulation of C57BL/6J mice.The number of 2-cell embryos is maximized when 10 IU PMSG/hCG is used in the embryo transplantation.关键词
C57BL /6J小鼠/超数排卵/胚胎移植/生物净化Key words
C57BL/6J mouse/Superovulation/Embryo transplantation/Purification分类
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冯颖,王芊芊,陆文昊,曹敏梁..利用超数排卵技术进行优化 C57 BL／6 J小鼠的生物净化体系[J].中国比较医学杂志,2014,(6):12-15,4.
