中国比较医学杂志Issue(6)：30-34,5.DOI:10.3969/j.issn.1671.7856.2014.006.007
长爪沙鼠动情周期血清激素水平与卵泡发育的动态研究
Dynamic study on levels of serum PRL, LH, FSH, E2 and development of ovarian follicles in different phases of estrus cycle in gerbils
摘要
Abstract
Objective To investigate the numbers of corpus luteum and ovarian follicles and compare the levels of serum prolactin (PRL), luteinizing hormone (LH), follicle-stimulating hormone (FSH) and estradiol (E2 ) in different phases of estrus cycle in female gerbils .Methods Consecutively taking vaginal smears of the gerbils and directly examined under light microscope to distinguish the four phases of the estrus cycle .Hematoxylin and eosin staining was used to histological examination of the gerbil ovaries , and to detect the levels of serum PRL , LH, FSH and E2 by ELISA assay during estrus cycle .Results The proportion of cornified vaginal exfolliated cells could be the basis to distinguish four phases respectively:proestrus, oestrus, metoestrus, and dioestrus.Moreover, there were no significant differences between the numbers of ovarian follicles in different phases of estrus cycle .The numbers of corpus luteum in preoestrus were significantly lower than that in the other phases of estrus cycle ( P <0.05 ) .The levels of serum PRL and LH were increasing constantly from preoestrus to dioestrus , and both reached a peak at dioestrus ( P<0.05 ) .The levels of serum FSH and E2 both peaked at preoestrus , and were significantly higher than those at oestrus , metoestrus and dioestrus ( P<0.05).Conclusions There are no significant differences between the numbers of ovarian follicles in different phases of estrus cycle .Gonadotropin , prolactin and estradiol paly important roles in the regulation of estrous cycle .The phases during which surges of FSH and E 2 occur in Mongolian gerbils are similar to those of rats and mice , while the PRL and LH are different .Our findings provide further reference to the study of reproductive physiology of Mongolian gerbils .关键词
动情周期/长爪沙鼠/卵泡/催乳素/雌二醇/促卵泡刺激素/黄体生成素Key words
Estrous cycle/Mongolian gerbil/Ovarian follicle/Prolactin/Estradiol, E2/Follicle-stimulating hormone , FSH/luteinizing hormone , LH分类
医药卫生引用本文复制引用
肖丽,陈立青,戴方伟,宋晓明,郭红刚,卢领群,吕宇,萨晓婴..长爪沙鼠动情周期血清激素水平与卵泡发育的动态研究[J].中国比较医学杂志,2014,(6):30-34,5.基金项目
科技部“十二五”科技支撑计划项目子项目（2011BAI15B01-31，2013BAK11B01-41），省医药卫生平台研究计划重点项目（2012ZDA010），浙江省科技计划项目（2012C37097）。 （）
