中国比较医学杂志Issue(6)：54-57,4.DOI:10.3969/j.issn.1671.7856.2014.006.012
北京地区实验动物中嗜肺巴斯德杆菌的表型分析
Phenotypic analysis of Pasteurella pneumotropica in laboratory animals in Beijing area
摘要
Abstract
Objective To improve the accuracy of detection through analyzing the phenotypes of P.pneumotropica isolates in laboratory animals in Beijing area .Methods 306 suspicious P.pneumotropica strains were identified by biochemical identification and 16S rDNA sequencing.Then, to obtain the phylogenetic relationships combined with colony characteristics on blood agar plates and biochemical characteristics of 53 biotypes .Results BD Phoenix 100 automated bacterial identification system and 16S rDNA sequencing identified P.pneumotropica positive rate of 306 isolates were 164/306 and 227/306, respectively.There were 140 phenotypes in 227 true-positive strains, of which 106 were biotype Heyl and 23 were biotype Jawetz .Conclusions In the samples of laboratory animals in Beijing area , P.pneumotropica infection mainly are of biotype Heyl , and less is of biotype Jawetz .The phenotypes are diverse and widely distributed .关键词
嗜肺巴斯德杆菌/表型/细菌鉴定/16S核糖体DNAKey words
Pasteurella pneumotropica/Biotype/, Bacteria identification/16S rDNA/Laboratory animals分类
医药卫生引用本文复制引用
邢进,冯育芳,岳秉飞,贺争鸣..北京地区实验动物中嗜肺巴斯德杆菌的表型分析[J].中国比较医学杂志,2014,(6):54-57,4.基金项目
中国食品药品检定研究院中青年发展研究基金项目（编号2012B8）。 （）
