中国比较医学杂志Issue(8)：70-74,5.DOI:10.3969.j.issn.1671.7856.2014.008.016
CRISPR/Cas9系统：构建非人灵长类动物疾病模型的新技术
CRISPR/Cas9 system：a new gene modification tool for establishing disease models in non-human primates
摘要
Abstract
Animal models are highly valuable systems that have been extensively used to elucidate human disease pathogenesis and to find therapeutic ways to treat human diseases .Since non-human primates are close to humans,monkeys are important model species in exploring the mechanisms and treatment of human neurodegenerative diseases , neuropsychiatric disorders, cognitive function, and neural circuits.However, due to the lack of embryonic stem cell lines in large animals, the traditional gene targeting technology is difficult to establish primate animal models of human diseases . CRISPR/Cas9, as a recently developed tool for genome modifications , has been successfully used to target genomic loci in mouse, rat, monkey, and other species.Here, we discuss the utilization of CRISPR /Cas9 technology in establishing monkey models for studying human neurodegenerative diseases .关键词
CRISPR/Cas9 系统/非人灵长类动物/神经退行性疾病Key words
CRISPR/Cas9 system/Non-human primates/Neurodegenerative diseases分类
医药卫生引用本文复制引用
杨伟莉,涂著池,李晓江..CRISPR/Cas9系统：构建非人灵长类动物疾病模型的新技术[J].中国比较医学杂志,2014,(8):70-74,5.基金项目
科技部国家重大科学研究计划项目（课题编号2012CBA01304）和分子发育生物学国家重点实验室经费资助。 （）
