中国比较医学杂志Issue(9)：1-4,4.DOI:10.3969/j.issn.1671.7856.2014.009.001
胃癌相关mir-148a靶向调控胃泌素受体CCKBR
Gastric cancer related miR-148 a targets gastrin receptor CCKBR
摘要
Abstract
Objective To investigate the regulation role of gastric cancer related miR-148a on gastrin receptor CCKBR expression, and find the correct binding sites of miR-148a in CCKBR 3’UTR.Methods The potential binding sites of miR-148a in the CCKBR 3’UTR were predicted with the bioinformatic tools;The miR-148a expressing plasmid was constructed by PCR, and miR-148a expression was verified by Northern Blot;The luciferase report plasmids containing the wild type and mutated binding sites of CCKBR 3’ UTR were constructed, and were used to study the regulation mechanism and identify the binding sites of miR-148a by luciferase activity analysis; The regulation effect of miR-148a on CCKBR protein expression was checked by Western Blot.Results Three potential binding sites of miR-148a in the CCKBR 3’ UTR were found; The miR-148a expressing plasmid was constructed successfully, and highly expressed miR-148a after transfected to gastric cancer cells;The inhibitory effect of miR-148a on CCKBR protein expression was checked by Western Blot.Over-expression of miR-148a inhibited CCKBR expression by directly binding to the binding site in CCKBR 3’UTR 423bp.Conclusion CCKBR is a target of miR-148a, and its expression is inhibited by the binding of miR-148a on its 3’ UTR, indicating that miR-148a may participates in the progression of gastric cancer by regulating CCKBR expression.关键词
胃泌素受体/胆囊收缩素B受体/miR-148a/胃癌/microRNAKey words
Gastrin receptor/Cholecystokinin B receptor/miR-148a/Gastric cancer/microRNA分类
医药卫生引用本文复制引用
郭水龙,朱圣韬,李鹏,王拥军,王民,邢洁,郭庆东,孙秀梅,张澍田..胃癌相关mir-148a靶向调控胃泌素受体CCKBR[J].中国比较医学杂志,2014,(9):1-4,4.基金项目
国家重点基础研究发展计划（2012CB526600）；北京市科委课题（Z121100007512011）。 （）
