中国比较医学杂志Issue(9)：5-8,13,5.DOI:10.3969/j.issn.1671.7856.2014.009.002
Alpha-亚麻酸对 HepG2细胞脂肪酸合成关键基因的影响
The effects of Alpha-linolenic acid on the related key gene expressions of fatty acid synthesis in HepG2 cells
摘要
Abstract
Objective To investigate the effects of different doses Alpha-Linolenic acid ( ALA ) on the expressions of fatty acid synthesis-related genes in HepG2 cells.Methods HepG2 cells were divided into two groups, normal control group (NC) and high fat group (HF) in which cells were firstly cultured for 36h in the medium contained 0.5mmol/L stearic acid.Real-time quantitative PCR was taken to detect mRNA expression of genes SREBP1C, FAS and ACC which are related to fatty synthesis.While there are significant differences in fatty synthesis, 10%, 20%, 50%, 70% and 100%ALA took the place of stearic acid, 36h later, real-time quantitative PCR and Western blotting were taken to detect mRNA and protein expression of genes related to fatty synthesis.Results SREBP1C mRNA expression of ALA substitution groups were significantly lower than the high-fat group ( P <0.001) .The FAS of 0.5 mmol/L ALA group and 0.35 mmol/L ALA group were significantly lower than the high-fat group (P <0.001).ACC genes mRNA level was not significantly different from high-fat group.SREBP1C and FAS protein expression were significantly lower than the high-fat group, but ACC showed no significant difference.Conclusions Saturated fatty acids promote hepatocyte fatty acid synthesis, ALA abates fatty acid synthesis by inhibiting FAS and SREBP1C gene expression.关键词
Alpha-亚麻酸/脂肪酸合成/基因表达Key words
Alpha-Linolenic acid/Fatty acid synthesis/Gene expression分类
医药卫生引用本文复制引用
李微,张涛,解现星,孟威,刘慧芳,肖霖力,李春风,刘源..Alpha-亚麻酸对 HepG2细胞脂肪酸合成关键基因的影响[J].中国比较医学杂志,2014,(9):5-8,13,5.基金项目
国家自然科学基金面上项目（31272386）。 （）
