中国比较医学杂志Issue(9)：40-43,4.DOI:10.3969/j.issn.1671.7856.2014.009.009
近交系小鼠移植性肝癌模型建立及对比分析
Establishment and comparative analysis of liver transplantation model in inbred mice
李晓娟 1汤紫荣 2李蓓 1李瑞生1
作者信息
- 1. 解放军第302医院实验技术研究保障中心，北京 100039
- 2. 解放军第302医院感染控制科，北京 100039
- 折叠
摘要
Abstract
Objective To select a best mouse strain for establishing an animal model of orthotopic transplanted liver cancer through comparison analysis.Methods C57,C3H and BALB/c mice( each 10) were selected as model groupⅠ,Ⅱ and Ⅲ.H22 cells were inoculated into the liver parenchyma of mice in model group along the ventral midline laparotomy.Fifteenth days after injection of laparotomy,the rate of tumor was observed,the volume of ascites and gross tumor volume were measured,and tumor pathology was analyzed.Results The survival rate of mice in three groups was 100%. After 15 days,ascites in three groups were produced,but with no significant difference among the three groups of the volume of ascites.Tumor transplantation rate in model groupⅠwas 100%,which was higher than 60%in model groupⅡand 30%in model group Ⅲ.Liver tumors in model group Ⅰ were all large compact grayish white lesions, and the average tumor volume was significantly greater than that of model group Ⅱ and Ⅲ( P <0.05 ) .Pathology results confirmed that white lesions in liver of three groups were hepatocelluar carcinoma in situ.Conclusion C57 is the better ideal experimental animal for establishing orthotopic liver caner model,and these results will provide a good experimental platform for research in pathogenesis of hepatocellular carcinoma.关键词
近交系小鼠/移植性肝癌/模型,动物Key words
Inbred mice/Transplanted liver cancer/Model,animal分类
医药卫生引用本文复制引用
李晓娟,汤紫荣,李蓓,李瑞生..近交系小鼠移植性肝癌模型建立及对比分析[J].中国比较医学杂志,2014,(9):40-43,4.
