中国比较医学杂志Issue(11)：6-9,4.DOI:10.3969/j.issn.1671.7856.2014.011.002
猕猴B病毒gB蛋白胞外区基因片段的合成和真核表达
Synthesis and eukaryotic expressing of the gene encoding extracellular domains of gB protein B virus
摘要
Abstract
Objective To get the gene encoding extracellular domains of gB protein of B virus and analyze its expression in the eukaryocyte cell.Methods synthesizing gene fragment encoding extracellular domains of gB protein of B virus was by using synthesis gene, then digested with the restriction endonucleases BamHⅠand NotⅠand inserted into eukaryotic expressing vector pEGFP-N3.pEGFP-N3-GB合 was transfected into 293 cells.After protein extraction, the expression of gene was detcted by western blotting, and the cellular localization of the gene was analyzed by immunofluorescence and laser scanning confocal microscopy.Results pEGFP-N3-GB合were expressed in 293 cells and on the cell membrane.Conclusion eukaryotic expressing system can produce specific antigen recombination protein of B virus gB protein and express on the cell membrane.关键词
猕猴B病毒/gB蛋白/合成/真核表达Key words
B virus/gB protein/Synthesis/Eukaryotic expressing分类
医药卫生引用本文复制引用
刘慧芳,孙书芳,曾林,易思萌,游颖,马雨楠,范君文,孙兆增,王新..猕猴B病毒gB蛋白胞外区基因片段的合成和真核表达[J].中国比较医学杂志,2014,(11):6-9,4.基金项目
国家科技支撑计划（2011BAI15B03）；十二五重大传染病专项（2011ZX09201-031）。 （）
