中国比较医学杂志Issue(11)：10-14,5.DOI:10.3969/j.issn.1671.7856.2014.011.003
比格犬 ERβ剪切异构体 ERβ419沉默表达载体的构建及筛选
Construction and identification of ERβ419 gene silencing recombined vectors of beagle ERβisoforms
甘艺 1赵彦斌 2陈福军 3孙兆增 4胡仲明 3刘冰 3杨焕民 3曾林1
作者信息
- 1. 黑龙江八一农垦大学动物科技学院，黑龙江大庆 163319
- 2. 实验动物中心，北京 100071
- 3. 实验动物中心，北京 100071
- 4. 五棵树经济开发区动物卫生监督所，长春 130401
- 折叠
摘要
Abstract
Objective To construct and identify retroviral-mediated short hairpin RNA ( shRNA ) expression vectors of ERβ419, and explore ERβ419 unknown biological function in beagles in future.Methods To screen out the most effective gene silencing sequence of beagle ERβ419 mRNA using qRT-PCR and Western Blot assays, imitate beagle estrogen target cells.Results qRT-PCR results showed, ERβ419-shRNA1 ( P <0.01 ) and ERβ419-shRNA3 ( P <0.01)differed significantly, Western Blot result as same as qRT-PCR,ERβ419-shRNA3 is the best choice.Conclusion Beagles ERβ419-shRNA3 retrain most effectively target gene repression. It is applied to explore ERβ419 unknown biological function in beagles reproductive system, and to prevent and treat beagles reproductive function diseases.关键词
比格犬ERβ419/稳定表达细胞系/基因沉默Key words
Beagles ERβ419/Stable expression cell lines/Gene silencing分类
医药卫生引用本文复制引用
甘艺,赵彦斌,陈福军,孙兆增,胡仲明,刘冰,杨焕民,曾林..比格犬 ERβ剪切异构体 ERβ419沉默表达载体的构建及筛选[J].中国比较医学杂志,2014,(11):10-14,5.
