中国比较医学杂志Issue(11)：45-48,4.DOI:10.3969/j.issn.1671.7856.2014.011.009
黑线仓鼠及其白化突变系肌张力异常蛋白变体 X1基因的克隆与序列分析研究
Cloning and sequence analysis of dystonin(Dst) variant X1 in Cricetulusbarabensis and albino mutant Cricetulusbarabensis
易思萌 1刘慧芳 2曾林 1孙兆增 2王新1
作者信息
- 1. 军事医学科学院实验动物中心，北京 100071
- 2. 青岛农业大学，山东青岛 266109
- 折叠
摘要
Abstract
Objective To clone and sequence the dystonin variant X1 gene of Cricetulusbarabensis and the albino mutant Cricetulusbarabensis so as to find out the difference of encoding arear of the muscular ribosome between Cricetulusbarabensis and the albino mutant Cricetulusbarabensis.Methods According to the same type of abnormal muscle tone protein of the mice and rats, we designed 6 pairs of primers, and got their cDNA genes from skins of the Cricetulusbarabensis and the albino mutant Cricetulusbarabensis by RT-PCR amplification, then cloned and sequenced. Results Sequence alignment showed 17 variances in coding areas, and 24 in amino acid, but no in key nucleic acid and protein.Conclusion The variances in coding areas will not lead to the albino, and its mechanism requires further investigation.关键词
黑线仓鼠白化突变系/肌张力异常蛋白/克隆/序列比较Key words
Albino mutant Cricetulusbarabensisi/Dystonin/Cloning/Sequence analysis分类
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易思萌,刘慧芳,曾林,孙兆增,王新..黑线仓鼠及其白化突变系肌张力异常蛋白变体 X1基因的克隆与序列分析研究[J].中国比较医学杂志,2014,(11):45-48,4.
