中国比较医学杂志Issue(12)：47-54,8.DOI:10.3969/j.issn.1671.7856.2014.012.010
猫疱疹病毒Ⅰ型实时荧光定量 PCR方法的建立及初步应用
Establishment and preliminary application of a real-time fluorescent quantitative PCR assay for detection of feline herpesvirus 1
摘要
Abstract
Objective To establish a real-time fluorescent quantitative PCR ( Q-PCR) method for detection of feline herpesvirus 1 ( FHV-1 ) in experiment cats and clinical sick cats.Methods Primers and TaqMan probes were designed and synthesized according to the published FHV-1 specific sequences of TK gene.FHV DNA standards were prepared using molecular biological techniques.The linearity, specificity, sensitivity, stability of the established Q-PCR method were tested.The method was used to detect 48 samples of cats.Results The linear range was 102 copies/μL to 109 copies/μL.The developed Q-PCR method showed no cross reaction with herpes virus type 1 ( HSV-1 ) , canine herpesvirus (CHV), pig pseudo rabies virus (PRV) and cat parvovirus (FPV).The sensitivity was 10 copies/μL.The coefficient of variation ( CV ) was less than 5%.There were 33 positive cases detected in the 48 samples of cats. Conclusions The developed Q-PCR method is good in linearity, specificity, sensitivity, stability, and may be used for rapid quantitative detection of FHV-1 in cats.关键词
猫疱疹病毒Ⅰ型/实时荧光定量PCR/猫Key words
Feline herpesvirus 1/Real-time fluorescent quantitative PCR/Cat分类
医药卫生引用本文复制引用
王吉,卫礼,付瑞,李晓波,王淑菁,巩薇,岳秉飞,贺争鸣..猫疱疹病毒Ⅰ型实时荧光定量 PCR方法的建立及初步应用[J].中国比较医学杂志,2014,(12):47-54,8.基金项目
实验动物质量检测关键技术研究（国家科技支撑计划项目2013BAK11B01）。 （）
