中国比较医学杂志Issue(4)：18-22,5.DOI:10.3969/j.issn.1671.7856.2015.004.004
C57－ras 转基因小鼠模型杂交1代C B6 F1的生长发育特性
Characterization of growth performance of F1 hybrid CB6 F1 C57-ras transgenic mouse model
摘要
Abstract
Objective To obtain the basic growth parameters of a self-established F1 hybrid CB6F1 C57-ras transgenic mouse model, and to provide basic information for commercialization of this mouse model. Methods F1 hybrid mice (CB6F1) were produced by crossing C57-ras heterozygous transgenic (c-Ha-ras+/-) male mice and wild-type BALB/cJ female mice.The average litter size, weaning rate, sex ratio, growth performance and C57-ras transgenic positive rate were recorded and analyzed.Results The average litter size was eight, weaning rate was 90%, and sex ratio was approximately in accordance with prediction.The average body weight of newborn mice was 1.73 ±0.05 g.The average body weight of 10-week old c-Ha-ras transgenic female and male mice in CB6F1 background was 24.38 ±1.74 g and 29.42 ±1.72g, respectively, which had a significant difference (P<0.01).The c-Ha-ras transgenic positive rate was 46.9%. which was in accordance with genetic rules.Conclusions The F1 hybrid mice (CB6F1) produced by crossing C57-ras heterozygous transgenic ( c-Ha-ras +/-) male mice and wild-type BALB/cJ female mice show normal growth performance and development characteristics, and it can be used for large-scale commercial supply.关键词
C57-ras转基因小鼠/人原癌基因c-Ha-ras/CB6F1/生长发育特性Key words
C57-ras transgenic mice/Human c-Ha-ras gene/CB6F1/Growth performance分类
医药卫生引用本文复制引用
刘甦苏,吴曦,周舒雅,王辰飞,彭泽旭,左琴,李保文,贺争鸣,范昌发..C57－ras 转基因小鼠模型杂交1代C B6 F1的生长发育特性[J].中国比较医学杂志,2015,(4):18-22,5.基金项目
中国食品药品检定研究院中青年发展研究基金（批准号2010C4）资助的课题。 （）
