中国比较医学杂志Issue(5)：13-19,7.DOI:10.3969/j.issn.1671.7856.2015.005.004
大鼠原发免疫性血小板减少性紫癜热盛模型的建立与评价
Establishment and evaluation of heat sheng model of rat primary immune thrombocytopenic purpura
摘要
Abstract
Objective To establish a rat model at the same time in accordance with the “hot sheng syndrome” of traditional Chinese medicine and primary immune thrombocytopenic purpura of peripheral blood platelet reduction. Methods Using back multi-point injection of 20% dry yeast suspension on SD rats and 1∶4 dilution of rabbit anti SD rats platelet serum (APS) by intraperitoneal injection to establish a primary immune thrombocytopenic purpura “heat sheng”rat model.And observing rats of TCM syndrome characteristics, hemogram, myelogram and serotonin (5-HT) level of the temperature regulating center in thalamus.Results After injection of 2 h ~6 h temperature and daily water of the model group rats increased significantly,toe purper showed in fourth day of modeling and intestinal mucosal bleeding in thirty day of modeling(P <0.05);Platelet count in peripheral blood decreased significantly, bone marrow megakaryocyte number reduced significantly((P <0.05);5-HT level of the temperature regulating center of brain increased significantly((P <0.05).Conclusions The study of the primary immune thrombocytopenic purpura heat sheng rat model of combination of disease and syndrome reflected basically the pathological characteristics of purpura caused by “heat sheng” in primary immune thrombocytopenic purpura rat mode.关键词
原发免疫性血小板减少性紫癜/热盛证/大鼠模型Key words
Primary immune thrombocytopenic purpura/Heat sheng/Heat syndrome/Rat model分类
医药卫生引用本文复制引用
聂甜,蒋文明,彭素娟,张旻昱,杨琳,李仕能,胡妮..大鼠原发免疫性血小板减少性紫癜热盛模型的建立与评价[J].中国比较医学杂志,2015,(5):13-19,7.基金项目
湖南省研究生科研创新项目（CX2013B331）。 （）
