中国比较医学杂志Issue(5)：58-61,4.DOI:10.3969/j.issn.1671.7856.2015.005.014
光激化学发光免疫分析技术在仙台病毒检测中的应用
Development of an amplified luminescent proximity homogeneous assay for detecting Sendai virus
摘要
Abstract
Objective To establish the amplified luminescent proximity homogeneous assay(AlphaLISA) for the detection of Sendai virus.Methods The antigen concentration,serum concentration and the donor beads/acceptor beads ratio used in the AlphaLISA method were optimumized by the phalanx experiments, then the antibodies of Sendai Virus in 40 rat sera were detected by the established AlphaLISA method and ELISA detection method.The results were compared and the differentia between the two methods was confirmed by IFA.Results The optimum concentration of SV bio-peptide in AlphaLISA assay was 250 nmol/L, the best proportion of donor beads/acceptor beads ratio was 1 ∶1, using the concentration of 20 μg/mL and the serum dilution was 1∶10000.7 of the 40 rat sera were detected SV positive by ELISA, the positive rate was 17.5%, 8 of the 40 rat sera were determined SV positive by AlphaLISA, and the positive rate was 20.0%, the AlphaLISA positive serum was confirmed by IFA.Conclusions We preliminary established the Amplified Luminescent Proximity Homogeneous Assay(AlphaLISA) for the detection of Sendai virus.The sensitivity of the method is comparable to classical ELISA method, but this method use less serum samples and without washing steps.The method has some advantages in degeneracy and accuracy.关键词
仙台病毒/AlphaLISAKey words
Sendai virus/AlphaLISA分类
医药卫生引用本文复制引用
常慧,高伟,张江义,向志光,魏强..光激化学发光免疫分析技术在仙台病毒检测中的应用[J].中国比较医学杂志,2015,(5):58-61,4.基金项目
协和青年基金和中央高校基本科研业务费专项资金资助（3332013042；33320140015）。 （）
