中国比较医学杂志Issue(6)：23-27,80,81,7.DOI:10.3969/j.issn.1671.7856.2015.006.005
维生素C对紫外线诱导的光老化大鼠皮肤结构的影响
Effects of vitamin C on UV-induced photoaged skin in rats
摘要
Abstract
Objective To study the protective effect of vitamin C on UV-induced photoaged skin structure in rats and provide a basis for clinical medicine and health care.Methods Photoaging skin models were set up by chronic ultraviolet radiation in 24 SPF female Sprague-Dawley rats, irradiated twice weekly for 4 weeks.The rat photoaged skin was induced by exposure to a total dose of 123 J/cm2 UVA and 5 J/cm2 UVB for 4 weeks.Vitamin C was administered by gastric gavage in a dose of 50 mg/kg once daily for 30 days during the model development.We compared the pathological changes in the irradiated skin using HE, Masson’ s trichrome and Victoria blue B staining, and compared the ultrastructural changes by electron microscopy.Results Rat models with photoaged skin were developed successfully.The vitamin C group showed significant reduction of pathological severity including erythema, ulcers, epidermal cell proliferation, epidermal thickness, dermis vasodilation, inflammatory cell infiltration, fibroblast proliferation, endoplasmic reticulum dilatation, mitochondrial swelling and vacuolization, elastic fibers degeneration and focal accumulation, collagen fibers swelling with uneven thickness,compared with the rats of model group at the irradiation site.Conclusions Vitamin C is effective in reducing the structural damage of UV-induced photoaged skin in rats.关键词
维生素C/抗光老化/皮肤结构/模型,大鼠Key words
Vitamin C/Anti-photoaging/Skin structure/Model rat/Histopathology/Pathology/Electron microscopy分类
医药卫生引用本文复制引用
楼彩霞,高擎,孙侠,黄威,钟志勇,邝少松,葛亚中..维生素C对紫外线诱导的光老化大鼠皮肤结构的影响[J].中国比较医学杂志,2015,(6):23-27,80,81,7.基金项目
国家自然科学基金（编号81403212）；广东省医学科研基金（编号B2014062）；广东省中医药管理局基金（编号20142019）。 （）
