中国比较医学杂志Issue(6)：32-35,83,5.DOI:10.3969/j.issn.1671.7856.2015.006.007
靶向脐带间充质干细胞的构建及其在小鼠脾脏内的定位
Construction of targeted umbilical cord derived mesenchymal stem cells and their distribution in the mouse spleen
摘要
Abstract
Objective To construct lentiviral vectors containing peptide P1-GFP fusion genes.Umbilical cord derived mesenchymal stem cells were infected with lentivirus carrying peptide P1 and GFP fusion genes.To inject the targeted umbilical cord derived mesenchymal stem cells into mice and to detect GFP expression in the spleen.Methods Umbilical cord derived mesenchymal stem cells were cultured with adhered tissues of umbilical cord smaller than 1 mm3 . Lentiviral vector containing P1-GFP fusion genes with engineering technology was constructed and infected the umbilical cord derive mesenchymal stem cells.Targeted umbilical cord derived mesenchymal stem cells were intravenously injected in the mouse tail vein and after 18 hours GFP expression was detected with immunohistochamical staining of the spleen tissues.Results Harvested umbilical cord derived mesenchymal stem cells grew well in culture medium. Green fluorescence on umbilical cord derived mesenchymal stem cells were observed under fluorescence microscope at 18 hours after infected with lentivirus.Green fluorescence intensity of umbilical cord derived mesenchymal stem cells was increasing over time and reached a peak at 72 hours.Umbilical cord derived mesenchymal stem cells highly expressed CD105 (90.0%)/CD44 (98%) and CD73 (85.0%)/CD90 (98.5%) molecules.GFP expression was detected in the spleen after intravenous injection of targeted umbilical cord derived mesenchymal stem cells in the mice 18 hours later.GFP expressing cells intimately contacted with lymphocytes.Conclusions Targeted umbilical cord derived mesenchymal stem cells contain P1-GFP fusion genes are constructed.Targeted umbilical cord derived mesenchymal stem cells can be targeted to mouse spleen and intimately contact with lymphocytes after intravenous injection.Our results lay the groundwork for further studies.关键词
靶向/脐带间充质干细胞/慢病毒/脾脏Key words
Targeted umbilical cord derived mesenchymal stem cells/Lentivirus/Spleen/Mice分类
医药卫生引用本文复制引用
秦力维,张宁坤,路平,彭秀军,王桂琴,高原,曹利群,崔蓓,郭建巍..靶向脐带间充质干细胞的构建及其在小鼠脾脏内的定位[J].中国比较医学杂志,2015,(6):32-35,83,5.基金项目
国家自然科学基金（30872394）及海军总医院创新培育基金资助（CXPY201312）。 （）
