中国比较医学杂志Issue(7)：25-29,5.DOI:10.3969/j.issn.1671.7856.2015.007.006
衰老对小鼠肺干细胞修复能力的影响
Effect of aging on repair capability of lung stem cells
摘要
Abstract
Objective To study the impact of aging on the capability of lung stem cell steady-state maintaining and bronchial epithelial cells regeneration and differentiation during the repair of lung epithelial cells after naphthalene induced bronchial epithelialium injury.Methods The proportion of lung stem cells in mice after naphthalene treatment was analyzed by immunohistochemistry and FACS.The repair efficiency of lung epithelial cell in young and old mice was examined by immunohistochemistry staining and FACS.Results The data suggested that aging didn ’ t change the proportion of lung stem cells ( including the distal lung epithelial stem cells/progenitor cells and lung mesenchymal stem cells/progenitor cells) under normal physiological conditions.After naphthalene injury, more serious injury and decreased repairing capacity was observed in old group.Lung progenitor cells /total lung cells decreased during the repair process of lung bronchial epithelialium ( clara cell) injury.The ratio of regenerated cell to lung progenitor and stem cells were also significantly decreased in old group.Conclusion The regenerated capability of lung stem cells after lung bronchial epithelialium injury decreased with aging.This might be the reason of more incidence of lung injury and worse therapeutic results in the elder in clinic.关键词
肺损伤修复/衰老/流式细胞术/肺干细胞Key words
Lung damage and repair/Aging/FACS/Lung stem cell分类
医药卫生引用本文复制引用
朱栩栋,姚超,龚松迪,何立锋,李晓燕,张玲玲..衰老对小鼠肺干细胞修复能力的影响[J].中国比较医学杂志,2015,(7):25-29,5.基金项目
国家自然科学基金（81300264）；2012年度浙江省大学生科技创新活动计划（新苗人才计划2012R4210007）。 （）
