中国比较医学杂志Issue(7)：30-33,4.DOI:10.3969/j.issn.1671.7856.2015.007.007
Lewis 大鼠急慢性期风湿性心脏炎模型的比较
A comparative study of different animal model of acute and chronic rheumatic carditis in Lewis rats
摘要
Abstract
Objective To make comparisons of the three models of acute and chronic rheumatic carditis to find out an optimal animal model.Methods AntigenⅠwas a emulsifier mixed by complete freund’ s adjuvant( CFA) and Group A streptococcus(GAS).AntigenⅡwas mixed by incomplete freund’s adjuvant(IFA) and GAS.Female Lewis rats were randomly divided into four groups: A, B, C treatmeat groups were immuned with antigenⅠat the foot pad firstly. Subsequently, rats in group A、B、C were injected antigenⅠ, antigenⅡand activated GAS respectively to make the models of RHD.Rats in control group D were immunized with the same protocol outlined as treatment groups but without GAS. Respectively 7, 12, 24 weeks the rats were sacrificed 24 ( each group was 6).The blood biochemical item and Hematoxylin-eosin( HE) staining of hearts were detected.Results In group C the mortality was 25%.In group A, the incidence of carditis was the highest.Histopathological manifestations of group A, C was not only revealed acute damage such as inflammatory cell infiltrate as well as group B, but also the Aschofflike cells in the myocardial cells interstitial.But in group A and C there had a great degree of the inflammatory cells infiltration than group B.At 24th week rats in group A detected the rate and degree of valve fibrosis in chronic damage were higher than group B and C.None of rats in group D presented carditis or valvulitis.Conclusion In group A, giving the GAS with continuous stimulation after using the mixed emulsification of CFA and GAS to immune Lewis rats for five times was a appropriate method which could provide an optimal animal model for experimental study of acute and chronic rheumatic heart disease.关键词
A族溶血性链球菌/Lewis大鼠/风湿性心脏炎/风湿性心脏病Key words
Group A streptococcus/Lewis rat/Rheumatic carditis/Rheumatic heart disease分类
医药卫生引用本文复制引用
李文婷,曾志羽,桂春,郑慧蕾,李靖,韦恒,文宏,黄伟强..Lewis 大鼠急慢性期风湿性心脏炎模型的比较[J].中国比较医学杂志,2015,(7):30-33,4.基金项目
国家自然科学基金（81260067）。 （）
