中国比较医学杂志Issue(7)：59-63,5.DOI:10.3969/j.issn.1671.7856.2015.007.012
PMSG 和 hCG 诱导长爪沙鼠超数排卵方案的优化
The optimization scheme of Mongolian gerbils superovulation induced by PMSG and hCG
摘要
Abstract
Objective To optimize the scheme of Mongolian gerbil superovulation.Methods Based on the analysis of the animal-week-old, the dose and time interval of hormone, we got the best animal-week-old, dose and time interval of hormone for Mongolian gerbil superovulation.Results The 6 week old female Mongolian gerbils which injected of 10 IU PMSG and followed by 10 IU hCG in 70 hours later could get the best superovulation.We collected eggs at 16 hour after mate with male gerbils.The ovum pick-up rate reached 80%, the number of oocytes were 32.6 ±3.0, the number of the fertilized egg developed to 2-cell are 24.8 ±5.4.Conclusions This study summarizes the optimization scheme of Mongolian gerbil superovulation induced by PMSG and hCG and it supported the foundation for Mongolian gerbils embryo biotechnology.关键词
长爪沙鼠/超数排卵/孕马血清促性腺激素/人绒毛膜促性腺激素Key words
Mongolian gerbil/Superovulation/PMSG/hCG引用本文复制引用
唐旺,李长龙,杜小燕,陈振文..PMSG 和 hCG 诱导长爪沙鼠超数排卵方案的优化[J].中国比较医学杂志,2015,(7):59-63,5.基金项目
国家科技支撑计划项目（2015BAI09B01；2011BAI15B01-32）；北京市自然基金重点课题（7141002）。 （）
