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脂肪干细胞分离纯化方法研究进展

刘琴 王丽平 陈芳 张宜

中国比较医学杂志Issue(7):69-73,5.
中国比较医学杂志Issue(7):69-73,5.DOI:10.3969/j.issn.1671.7856.2015.007.015

脂肪干细胞分离纯化方法研究进展

Progress in the isolation and purification methods of adipose-derived stem cells

刘琴 1王丽平 1陈芳 1张宜1

作者信息

  • 1. 解放军广州军区武汉总医院医学实验科,武汉 430070
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摘要

Abstract

Adipose-derived stem cells ( ASCs ) as potential seeded cells have been widely used in tissue engineering.Thus to obtain enough, high activity, high purity adipose-derived stem cells is the particular important premise of the application in tissue engineering.In this paper, the isolation and purification methods of ASCs were reviewed and the merit and demerit of different methods were compared in order to provide theoretical basis for safe and high-effective isolation and purification of ASCs.

关键词

脂肪干细胞/分离/纯化/组织块贴壁法/酶消化法/免疫磁珠分选法/密度梯度离心法

Key words

Adipose-derived stem cells/Isolation/Purification/Explants culture method/Enzymatic digestion method/Immunomagnetic beads sorting method/Density gradient centrifugation

分类

医药卫生

引用本文复制引用

刘琴,王丽平,陈芳,张宜..脂肪干细胞分离纯化方法研究进展[J].中国比较医学杂志,2015,(7):69-73,5.

中国比较医学杂志

OA北大核心CSTPCD

1671-7856

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