中国动物传染病学报Issue(3)：14-18,5.
鸭坦布苏病毒病活疫苗（FX2010-180P株）毒种保存期的研究
EVALUATION OF STORAGE LIFE OF MASTER SEED VIRUSES OF DUCK TEMBUSU VIRUS LIVE VACCINE FX2010-180P STRAIN
摘要
Abstract
Duck Tembusu virus (DTMUV) is a newly emerging pathogenic flavivirus causing severe decline of egg production in ducks. However, vaccine is not available for prevention of this infectious disease. An attenuated DTMUV strain FX2010-180P was developed through 180 passages of virulent FX2010 in chicken embryo fibroblasts (CEFs). To evaluate the storage life of the master seed viruses for production of live vaccine, primary seed and master seed batches were tested for purity, identity and titer after stored for 16 months at-80℃. All batches tested were free from bacteria, mycoplasma and exogenous viruses and viral titers did not decline significantly. To test the immunogenicity of seed viruses, ducks were inoculated intramuscularly with 101.5 and 102.5 TCID50 of vaccine viruses propagated in CEFs. Ducks vaccinated with 101.5 and 102.5 TCID50 of vaccine viruses gained 90%and 100%protection against FX2010 challenge.关键词
鸭坦布苏病毒病活疫苗/毒种/保存期Key words
Duck Tembusu virus live vaccine/master seed virus/storage life分类
农业科技引用本文复制引用
吴晓刚,高旭元,余磊,李雪松,闫丽萍,滕巧泱,李国新,李泽君..鸭坦布苏病毒病活疫苗（FX2010-180P株）毒种保存期的研究[J].中国动物传染病学报,2014,(3):14-18,5.基金项目
自然科学基金（面上）项目（31172332）；上海市科技兴农重点攻关项目（沪农科攻字（2012）第2-6号）；上海市科委“创新行动计划”基础研究重点项目（12JC1410600）；上海市科委“创新行动计划”现代农业领域重点科技攻关项目 （）
