中国动物传染病学报Issue(3)：61-68,8.
p53调控Nfkbiz基因表达的研究
THE REGULATORY ROLE OF P53 ON EXPRESSION OF NFKBIZ GENE
摘要
Abstract
The functional mechanism of p53 in inhibition of inflammation is not well-understood. Nuclear factor-κB (NF-κB) plays a causative role in inflammatory processes and regulated by many factors. IκBζencoded by Nfkbiz gene is a member of IκB family and regulates the activity of NF-κB in inflammation. Expression of Nfkbiz mRNA was analyzed in quantitative Real-time PCR. The results showed that expression of Nfkbiz was significantly decreased in response to a specific p53 stimulus. Alignment of Nfkbiz gene with p53 response element revealed a potential p53 response element in the Nfkbiz gene. We conducted a luciferase report plasmid containing a potential p53 response element and found that Nfkbiz gene was negatively regulated by p53 protein. The binding of p53 to Nfkbiz gene was detected in chromatin immunoprecipitation assay. These data indicated that Nfkbiz gene was a target gene negatively regulated by p53.关键词
p53蛋白/Nfkbiz基因/NF-κB/靶基因Key words
p53 protein/Nfkbiz gene/NF-κB/target gene分类
农业科技引用本文复制引用
杨逸凡,杜立中,朱紫祥,李满,魏建超,史子学,邵东华,李蓓蓓,马志永..p53调控Nfkbiz基因表达的研究[J].中国动物传染病学报,2014,(3):61-68,8.基金项目
国家自然科学基金项目 （）
