中国动物传染病学报Issue(4)：7-16,10.
猪繁殖与呼吸综合征病毒5'非翻译区一顶端茎环结构是病毒复制转录必需的顺式作用元件
A PROMINENT STEM-LOOP STRUCTURE IN 5' UNTRANSLATED REGION OF PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS IS ESSENTIAL FOR VIRAL REPLICATION AND TRANSCRIPTION
摘要
Abstract
The 5' untranslated region (UTR) of the genomic RNA is believed to be vital for the replication and transcription of Porcine reproductive and respiratory syndrome virus (PRRSV), yet its functional mechanism remains unclear. Based on infectious full-length cDNA clones pAPRRS and pSHE of type 1 and type 2 PRRSV, exogenous“at”was inserted between 5' UTR and ORF1 to form Nde I digestion site, then pTLNd4 and pSHEnde were obtained. After transfection and passage on cell monolayers, the infectiousness of pTLNd4 and pSHEnde was demonstrated. Afterwards, the 5' UTR chimeric mutants pTLV8 and pSHSP5 were obtained. Interestingly pTLV8 developed cytopathic effect (CPE) on MARC-145 cells while pSHSP5 was a lethal mutant. Comparison of the secondary structures of 5' proximal sequences revealed that a signifi cant change occurred in pSHSP5 but not in pTLV8. The prominent stem-loop structure remained functional in pTLV8 as TRS and AUG of ORF1 were located in the top loop. Taken together, the prominent stem-loop structure where TRS is located is one of the crucial cis- acting elements in regulating viral replication and transcription. Therefore, any changes that alter the stem-loop structure would be lethal for mutant viruses.关键词
猪繁殖与呼吸综合征病毒/5'非翻译区/嵌合突变体/转录调控序列/二级结构预测Key words
Porcine reproductive and respiratory syndrome virus (PRRSV)/5' untranslated region (5' UTR)/chimeric virus/transcription-regulating sequence (TRS)/secondary structure分类
农业科技引用本文复制引用
高飞,郑浩,姜一峰,李丽薇,郑海红,周艳君,韦祖樟,童光志..猪繁殖与呼吸综合征病毒5'非翻译区一顶端茎环结构是病毒复制转录必需的顺式作用元件[J].中国动物传染病学报,2014,(4):7-16,10.基金项目
国家自然科学基金（31300140）；863计划（2011AA10A208-2）；科研院所基本科研业务费 （）
