中国动物传染病学报Issue(4)：29-34,6.
H1N1亚型猪流感病毒HA和NA基因双重RT-PCR定型检测方法的建立与应用
DOUBLE RT-PCR METHODS FOR DETECTION OF HA AND NA GENES OF H1N1 SUBTYPE SWINE INFLUENZA VIRUS
摘要
Abstract
To develop a reliable diagnostic method for H1N1 subtype Swine influenza virus, double RT-PCR methods were developed using primers designed according to the sequences of HA and NA genes of H1N1 Subtype swine influenza virus in GenBank. The amplified gene fragments were 428 bp for HA and 678 bp for NA. The reaction conditions were optimized and minimal detectable RNA concentration was 2.9×10-5μg/μL. Meanwhile, the method had no reaction with H3N2 subtype Swine influenza virus, classical Swine fever virus and Porcine reproductive and respiratory syndrome virus. This method developed here is suitable for double gene typing detection and also for early diagnosis and epidemiological investigation of H1N1 subtype Swine influenza virus.关键词
H1N1亚型猪流感/双重RT-PCR方法/HA基因/NA基因Key words
H1N1 subtype Swine influenza virus/double RT-PCR/HA gene/NA gene分类
农业科技引用本文复制引用
温肖会,蔡春梅,魏文康,翟少伦,吕殿红,贾春玲,袁洁,黄忠,周秀蓉..H1N1亚型猪流感病毒HA和NA基因双重RT-PCR定型检测方法的建立与应用[J].中国动物传染病学报,2014,(4):29-34,6.基金项目
广东省科技计划社会发展项目（2010B080701024）；广东省科技计划特定任务项目（2011B060700075）；广州市科技计划项目 （）
